FastQCFastQC Report
Sun 19 Mar 2023
SRR4896068_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896068_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501632
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG216101.4391009248604185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130390.8683219324042109No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG65810.43825651025018114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28040.1867301709073861No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23080.15369944167412522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT571050.0117.965781
GGTATCA581750.0115.721298
GTGGTAT580100.0115.537936
GTATCAA584600.0115.233339
TGGTATC582850.0114.99287
AGCAGTG585050.0114.980892
AGTGGTA584150.0114.774135
CAGTGGT589800.0113.763574
GCAGTGG591600.0113.3929143
GTCGATA700.001057106941.42621145
AGGGGTA5000.037.7066465
CGGTCTG3100.035.078648145
GGGTATC5750.034.0494777
CGATAAG1703.0143801E-629.85124145
GAGGTAT5550.028.7438036
CGCTGTT4200.027.617474145
GGGGTAT6950.027.1270796
AGGTATC6000.026.5880177
ATGGGAT108500.025.57870925-29
ACATGGG548000.024.38266620-24
Produced by FastQC (version 0.11.5)