FastQCFastQC Report
Sun 19 Mar 2023
SRR4896067_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896067_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618260
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACA619350.0144.63762
AAGGTAC620000.0144.485961
GGTACAT220450.0144.237693
GGTACAG200050.0144.206863
GTACAGT71750.0143.481324
GTACAAG66500.0143.478684
GGTACAA190500.0143.335713
GTACATG71200.0143.26694
GTACAGA62700.0142.699574
GTACATT77200.0142.642684
GTACAGG65500.0142.46164
GTACAAT69100.0142.168994
GTACATA72850.0141.712624
TACAGGT22150.0140.315875
TACAGTA21550.0140.188375
TACATGG23500.0139.962365
TACAGTG24250.0139.517415
TACAGGA18900.0139.145145
TACAGTT22850.0137.919715
TACAGAT22500.0137.811195
Produced by FastQC (version 0.11.5)