FastQCFastQC Report
Sun 19 Mar 2023
SRR4896066_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896066_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360330
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGA360950.0144.670042
GACGGAT123200.0144.636933
ACGGATA40950.0144.223194
AAGACGG362150.0144.190671
ACGGAAT42350.0143.904364
GACGGAA108200.0143.792483
ACGGAGT43900.0143.775544
GACGGAG124700.0143.594453
CGGAAGT10000.0143.481725
ACGGATT42650.0143.401864
CGGATAG12900.0143.245795
ACGGATG39600.0142.91814
CGGATTA13050.0142.154565
CGGAGTA15550.0142.134935
CGGAGTT13150.0141.624635
CGGATAT13100.0141.611985
CGGAATG12450.0141.438725
CGGATTG13400.0141.145525
CGGATGA11650.0140.576875
CGGATAA12800.0140.401955
Produced by FastQC (version 0.11.5)