FastQCFastQC Report
Sun 19 Mar 2023
SRR4896066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360330
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG63181.7533927233369413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22650.6285904587461494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG15160.42072544611883556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCAGTGGCTCACGCCT9140.25365637054921875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8430.2339522104737324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGTGCGGTGGCTCACA6580.18261038492492992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGTGCGGTGGCTCA6290.17456220686592844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACG5380.14930757916354453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCTGTGGCTCACG5300.14708739211278551No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAGTGGCTCACGCCTG4880.1354314100963006No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4670.12960341908805817No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3760.10434879138567425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT117150.0104.0580441
GGTATCA118850.0102.846168
GTGGTAT118300.0102.527636
GTATCAA119050.0102.490699
TGGTATC119200.0101.75357
AGCAGTG119750.0101.709952
AGTGGTA119550.0101.2736745
CAGTGGT120250.0100.985594
GCAGTGG127200.095.467913
ATTGCGC907.473745E-540.277004145
GTTCGGA850.00273992634.116993145
CAGGGGT2051.1345037E-831.8286574
AGGGGTA1902.070683E-730.5257345
ACACCAA5050.028.71232145
TGCGGCT2456.4155756E-826.632143145
GGGGTAT2301.1000247E-625.216916
GCAACCT1450.001225057424.999521145
TACGCAC1350.024.70323250-54
TCTCGCA1350.024.70323270-74
CATGGGG33050.024.34900325-29
Produced by FastQC (version 0.11.5)