FastQCFastQC Report
Sun 19 Mar 2023
SRR4896065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570921
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193151.229533502957819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG135610.8632515575258081No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC57640.3669185146802417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATATCTGCTTCCGACACA40790.2596565963533494No Hit
GGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGC29570.18823352670185198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG29290.1864511328068057No Hit
TGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG26400.16805428153293514No Hit
GAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCT24190.15398610114703412No Hit
GCTTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTC22450.14290979622781794No Hit
ATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTT22410.1426551685285256No Hit
GTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTC22100.1406818038590101No Hit
AAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGT21530.13705335914409444No Hit
GCCTACATCCTGCGCGTGGACCCGGTCAACTTCAAGCTCCTGTCCCACTG21230.135143651399402No Hit
GACGACATCGGCGGCGCCCTGTCCAAGCTGAGCGAGCTGCACGCCTACAT21170.1347617098504635No Hit
GTGGACCCGGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCT20780.13227908978236336No Hit
TTCCCCGCCGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCT20640.1313878928348402No Hit
GGCGGCGCCCTGTCCAAGCTGAGCGAGCTGCACGCCTACATCCTGCGCGT20270.1290325866163862No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20000.127313849646163No Hit
GACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGG19210.12228495258513955No Hit
TTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGG18440.11738336937376227No Hit
GGACCCGGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGG18280.11636485857659297No Hit
TGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCC18220.11598291702765448No Hit
GAGCTGCACGCCTACATCCTGCGCGTGGACCCGGTCAACTTCAAGCTCCT18210.1159192601028314No Hit
AAGCCCGCCTTTGCTAAGCTGAGTGAGCTGCACTGTGACAAGCTGCATGT18180.11572828932836215No Hit
AACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGCGCTT17960.11432783698225436No Hit
ACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCC17890.1138822385084928No Hit
AATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGA17690.11260910001203116No Hit
TGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGA17650.11235447231273882No Hit
CCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACT17490.11133596151556953No Hit
TGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACC17400.11076304919216179No Hit
TGCACGCCTACATCCTGCGCGTGGACCCGGTCAACTTCAAGCTCCTGTCC17210.10955356762052326No Hit
TGTCCCACTGCCTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTC17200.10948991069570017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGGGCTTGGCCTTGAGA16930.10777117372547697No Hit
GAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCA16890.10751654602618463No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG16890.10751654602618463No Hit
AAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGT16720.10643437830419225No Hit
CCCACTGCCTGCTGGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACG16630.10586146598078451No Hit
GGTCACCCTGGCCGCGCGCTTCCCCGCCGACTTCACGGCCGAGGCCCACG16550.10535221058219986No Hit
TGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGT16370.1042063859353844No Hit
TGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGT16360.10414272901056133No Hit
AAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGC16220.10325153206303818No Hit
GGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGG16220.10325153206303818No Hit
GGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACA16090.10242399204033813No Hit
CGACTTCACGGCCGAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCG16030.10204205049139962No Hit
TACTCACTTTGGCAAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGA15910.10127816739352266No Hit
GTGATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCTGA15790.10051428429564567No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACATATCTGCTTCCGACAC15790.10051428429564567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT239650.0103.959921
AGCAGTG244350.0101.729382
GTGGTAT244400.0101.600146
AGTGGTA244550.0101.514645
GGTATCA247350.0101.238438
GTATCAA248150.0101.051699
TGGTATC246900.0100.659487
CAGTGGT246700.0100.554584
GCAGTGG314700.078.965113
CGGTCTG2400.066.45575145
ACGCTTA1650.065.921212
GACGGCG1700.055.440797
CCGTAGG1101.6734703E-952.7369772
TCGACGG1900.049.608075
TCGGCGA1501.0913936E-1148.3422282
ACGGCGC2050.045.9752858
GCCGTAG1703.8198777E-1142.652191
CGCTTAA1105.259575E-639.552733
TGGAAGA27350.039.495724145
AGGGGTA2450.038.4715655
Produced by FastQC (version 0.11.5)