FastQCFastQC Report
Sun 19 Mar 2023
SRR4896064_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896064_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445547
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG49141.1029139462278952No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27530.617892164014122No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12780.28683842557575295No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11830.265516320388197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6850.15374360056290357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT92300.0106.897331
GGTATCA93150.0106.400798
GTGGTAT92950.0105.537696
AGCAGTG94950.0103.84922
GTATCAA95150.0103.695479
TGGTATC95600.0102.6880657
CAGTGGT96100.0102.304674
AGTGGTA96550.0101.6776665
GCAGTGG97900.0100.423693
CGGTCTG1800.056.385307145
AACCGAG1602.0008883E-1145.3096241
AGGGGTA1807.094059E-1140.279745
GGGTATC1901.2914825E-1038.159757
ACCGAGA1951.6916601E-1037.18132
GGGGTAT2002.237357E-1036.251776
GCCCCAA3500.031.069456145
GTATCAC1958.74457E-626.0239899
TAACCCG459.120977E-725.77614115-119
CGCGAAA1350.023.6281390-94
CAGGGGT3102.4090696E-823.3882394
Produced by FastQC (version 0.11.5)