FastQCFastQC Report
Sun 19 Mar 2023
SRR4896063_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896063_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066087
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT914508.578099160762678No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT222622.0881973047227853No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT105200.9867862566563517No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCCGC33040.30991842129207087No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGC29230.2741802498295168No Hit
AACAACCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT27580.2587030889599066No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTC25120.23562804911794252No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGGC22870.2145228297502924No Hit
AACAACCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG21890.2053303342034937No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTACCCATGTACTCTGCGT19390.18188009046166026No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGCGGC18770.17606443001368557No Hit
AACAACCATGTACTCCGCGTTGATACCACTGCTTCCCATGTACTCTGCGT18200.17071777444054753No Hit
AACAACCATGTACCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT17330.1625570896183895No Hit
AACAACCATGTACTCTGCGTTGAGACCACTGCTTCCCATGTACTCTGCGT17010.1595554584194348No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT15420.14464110339962874No Hit
AACAACCATGTACTCTGCGTTGATACCACTGCTTCCCAGGAACCCGCCGT13790.1293515444799533No Hit
AACAACCATGTACTCTGCGTTGATACGACTGCTTCCCATGTACTCTGCGT13530.12691271913080263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCATG355100.0144.028494
CAACCAT541700.0143.749193
ACCATGT287050.0143.134035
CAACCAG278600.0142.741533
ACAACCA1082500.0142.724012
AACAACC1090900.0141.625051
AACCAGT97150.0141.346084
ACCAGGT31600.0138.73495
ACCAGTT28550.0137.819645
ACCAGAT27350.0137.507835
ACCAGTA31950.0136.98835
ACCATAG31900.0136.74875
CCAGTAT11400.0136.663016
ACCAATG34600.0136.549125
AACCAGA85000.0136.401294
ACCAAGT28350.0136.23595
ACCATGA29050.0136.195885
ACCATGG30950.0135.79535
AACCATA94350.0135.172444
ACCATTG34250.0134.770945
Produced by FastQC (version 0.11.5)