FastQCFastQC Report
Sun 19 Mar 2023
SRR4896063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066087
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG18537117.387980530669637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA278402.6114191430905733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49150.4610317919644457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTTGTTAGATCGGAAGAGCACA46580.43692494139784094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG45320.42510601855195684No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA38000.35644370487586846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC27630.2591720938347433No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20010.18769575090963495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG15750.14773653557355074No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG15090.14154567122570672No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG14770.13854404002675205No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG13200.12381728695688063No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12470.11696981578426527No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG11930.11190456313602923No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11390.1068393104877932No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11180.1048694900134792No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT11090.10402528123877319No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10770.10102365003981852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGT2750.052.724903145
AAGCAGT1081400.051.8545571
AGCAGTG1127900.049.705092
AGTGGTA1176000.047.6984335
GGTATCA1184950.047.641858
GTATCAA1185900.047.60989
GTGGTAT1188800.047.207046
TGGTATC1191700.047.092167
CAGTGGT1205750.046.531934
GCAGTGG1209150.046.347123
CCGGCTT20700.042.027096145
CCGGGTT6750.040.81298145
CGGTATC2350.040.104587
CGTGGTT7800.039.966152145
CGTGGGA8550.034.76452145
CCGTCTT44300.033.875454145
GCGGTAT2800.033.65926
CGGGCTC4800.033.227673145
AGCGGTA2850.033.070245
CGGTCTG3950.033.03649145
Produced by FastQC (version 0.11.5)