FastQCFastQC Report
Sun 19 Mar 2023
SRR4896062_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896062_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118860
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTACAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3230.2717482752818442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAA151.2297864E-4144.926397
AGTGGCT151.2297864E-4144.926397
AGGTGCA151.2297864E-4144.926397
AGATCAG253.702371E-8144.926387
AAGTTAC253.702371E-8144.926386
CAAGATC1750.0144.926385
ATATTCG100.0070938226144.926389
GTACAAG118950.0144.804532
TACAAGT38600.0144.550923
AGTACAA119200.0144.500841
TACAAGC7250.0143.926883
ACAAGTT12500.0143.766974
ACAAGTG13050.0143.260564
ACAAGAT11950.0143.107224
ACAAGTA12700.0142.644074
TACAAGA33650.0142.342253
TACAAGG40900.0142.091633
CAAGATG3800.0141.112535
CAAGTAT3650.0140.95585
AAGGTTA1750.0140.785636
Produced by FastQC (version 0.11.5)