FastQCFastQC Report
Sun 19 Mar 2023
SRR4896062_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896062_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118860
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1313011.046609456503448No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31312.634191485781592No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA16911.4226821470637725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCACGCCTGTAATCCCA4480.37691401648998824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGCAAAAAAAAAAAAAAAA4030.3390543496550564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3520.2961467272421336No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3220.270906949352179No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3210.2700656234225139No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2520.21201413427561835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2310.1943462897526502No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2230.18761568231532896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCAACTTTAAATTTGCC2070.17415446744068652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT1790.15059734141006226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTACGCCTGTAATCCCAGCA1730.14554938583207136No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1630.13713612653541982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCTCACACCTGTAATCCCA1540.12956419316843346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1530.1287228672387683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATGCAAAAAAAAAAAAAAA1450.12199225980144708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCCTTATGAAACTTAAGG1430.12030960794211679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATACTCAATTGATCCAATAA1410.11862695608278646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC1370.11526165236412586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1330.11189634864546526No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1310.11021369678613495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACGATAGCCCTTATGAAACT1310.11021369678613495No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC1300.10937237085646981No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCACCAGTCAAAGCGAACTAC1280.1076897189971395No Hit
AAGCAGTGGTATCAACGCAGAGCACACGTCTGAACTCCAGTCACTTGGCG1270.10684839306747435TruSeq Adapter, Index 20 (96% over 31bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1210.10180043748948342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAACAGCTAAGGACTGCAAA1210.10180043748948342No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1200.10095911155981827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA100.007085887144.98065145
GAATACC151.2279539E-4144.98064145
AGGGGTA502.253546E-886.9883965
GAGATTC353.427072E-582.846085145
AAGCAGT126650.082.684631
AGTGGTA128350.081.216275
CAGTGGT129400.080.725314
AGCAGTG129750.080.619292
GTGGTAT130250.079.975886
GTATCAA131600.079.706319
GGTATCA131400.079.6621258
GCAGTGG131350.079.4716953
TGGTATC131650.079.1804667
GGGTATC651.388762E-766.914157
CCGCAAG1150.063.035065145
CAGGGGT702.319066E-762.1345634
CACGGGA1051.0968506E-955.230724145
GCTGGCC450.00962348348.326885145
TATGGGA450.00962348348.326885145
AGAGAGT2000.047.118713145
Produced by FastQC (version 0.11.5)