FastQCFastQC Report
Sun 19 Mar 2023
SRR4896061_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896061_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741917
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT742900.0144.70032
AACGTGA743100.0144.661351
CGTGATA187300.0144.469863
CGTGATT198300.0144.237723
CGTGATG328200.0144.188963
GTGATGT151200.0141.924594
GTGATGA89750.0141.585754
GTGATAA56450.0140.660144
CGTGATC32150.0140.388493
GTGATTA58400.0140.305344
GTGATAG45650.0140.292954
GTGATGG90000.0139.341024
GTGATAT90100.0138.864734
TGATGAG34150.0138.106695
TGATGTG60350.0136.97235
GTGATTG48850.0136.886784
TGATTAG22150.0134.755945
TGATATG34700.0134.664985
TGATAAG22750.0134.386445
GTGATCA9350.0133.272724
Produced by FastQC (version 0.11.5)