FastQCFastQC Report
Sun 19 Mar 2023
SRR4896061_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896061_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences741917
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG197712.6648533461290143No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA115851.5614954233425034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGGACAGTGGCTCA30720.41406248946984636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28370.3823877873131361No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACTCGCTTCTGGAACGTC17860.24072773639099793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACATATCTGCTTCCGACACA13080.17630004434458302No Hit
GGTCAACTTCAAGCTCCTGTCCCACTGCCTGCTGGTCACCCTGGCCGCGC10400.14017740528927092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACACTCGCTTCTGGAACG10290.13869475965640363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGGGCTTGGCCTTGAGA10040.13532511049079615No Hit
TGAGTGAACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG9710.13087717359219428No Hit
GAGGCCCACGCCGCCTGGGACAAGTTCCTATCGGTCGTATCCTCTGTCCT8010.10796355926606345No Hit
GCCTACATCCTGCGCGTGGACCCGGTCAACTTCAAGCTCCTGTCCCACTG7750.10445912413383168No Hit
GTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCTGAGGTGCAGGCTTC7630.1028416925343401No Hit
AAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGT7630.1028416925343401No Hit
ATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTT7620.10270690656771579No Hit
GACGACATCGGCGGCGCCCTGTCCAAGCTGAGCGAGCTGCACGCCTACAT7560.10189819076797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT185500.079.6526951
AGTGGTA189000.077.85345
GTATCAA191400.077.305799
GGTATCA192600.076.995458
AGCAGTG192800.076.695082
CAGTGGT192950.076.334784
GTGGTAT193000.076.1226
TGGTATC194450.075.591667
GCAGTGG223850.065.7976153
CTCTACC7300.065.54387145
GGGGTAT1250.063.813236
ACGACTC805.860493E-754.3899423
AGGGGTA1650.048.346615
CGACTCC855.3315034E-542.658784
CGGTCTG1201.9734944E-742.289032145
ACGGCGC951.02737926E-438.1658028
TCGACGG1051.8502658E-434.5332955
TGTGGAA12700.034.249836145
CGGCGAC850.0027378434.1270263
CGAGCGC900.003628738732.220215145
Produced by FastQC (version 0.11.5)