FastQCFastQC Report
Sun 19 Mar 2023
SRR4896060_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896060_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257123
Sequences flagged as poor quality0
Sequence length151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG243229.45928602264286No Hit
CTGTAGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC66742.5956448859106342No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT23290.905792169506423No Hit
CTGTAGCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGC11960.4651470307984894No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCG11870.46164676049983855No Hit
CTGTAGCCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT9710.3776402733322184No Hit
CTGTAGCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC8980.3492491920209394No Hit
CTGTAGCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC8350.3247472999303835No Hit
CTGTAGCCTGTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC8190.3185245971772265No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCTGCG7340.2854664888010796No Hit
CTGTAGCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG7270.2827440563465734No Hit
CTGTAGCCCATGTACTCTGCGTTGATACCACTGCTTCGCACGTACTCTGC6950.27029865084025934No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACAGCTTCCCATGTACTCTGCG5850.22751756941230464No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACGCTGCG5120.19912648810102557No Hit
CTGTAGCCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCG4990.19407054211408548No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG4920.19134810965957927No Hit
CTGTAGCCATCTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4610.17929162307533747No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGT4360.1695686500235296No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGCG3890.15128946068613075No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCGAACGGACGCGCGG3690.14351108224468445No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACGCTGCG3470.1349548659590935No Hit
CTGTAGCCATGTACCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3160.12289837937485174No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTAGAACGGAAGAGCGG3050.11862027123205625No Hit
CTGTAGCCCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGG3020.1174535144658393No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCGCACGTACGCGGCG2970.11550891985547772No Hit
CTGTAGCCCATGTACTCTGCGTTGATACCACTGCTTCGCACGGACGCGGC2910.11317540632304382No Hit
CTGTAGCCGTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2800.10889729818024836No Hit
CTGTAGCCATGTACTCTGCGGTGATACCACTGCTTCCCATGTACTCTGCG2680.10423027111538057No Hit
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCAAGGACCCCGCGG2660.10345243327123593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA450.0144.930578
CTATGAG202.1326432E-6144.930578
ATCTAGG100.0070979767144.930579
GAGTAAG100.0070979767144.930579
TGAGTCA100.0070979767144.930579
CGTTAAG253.7141945E-8144.930578
CCGACAT202.1326432E-6144.930577
CGTAAGT202.1326432E-6144.930578
CGATAGT202.1326432E-6144.930578
CGATTAG151.2312981E-4144.930568
CTGTAGC257400.0144.761641
TGTAGCC257400.0144.761642
GTAGCCT67500.0144.60853
TAGCCAT70750.0144.520864
GTAGCCA105050.0144.516683
GTAGCCG61500.0144.459263
AGCCATG55850.0144.411585
TAGCCGT25350.0144.358844
TAGCCTA22000.0144.271794
TAGCCGA17700.0143.702354
Produced by FastQC (version 0.11.5)