FastQCFastQC Report
Sun 19 Mar 2023
SRR4896060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257123
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6427924.999319391886374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA88193.429875973755751No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA14460.5623767613165683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9490.3690840570466275No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7500.29168919155423667No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7340.2854664888010796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGCTACAGAGATCGGAAGAGCAC6350.2469635155159204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5800.22557297480194305No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5530.21507216390599052No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5050.19640405564651936No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4800.18668108259471147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG4270.16606837972487876No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4180.1625681094262279No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4150.16140135266001096No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4120.16023459589379402No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4100.15945675804964937No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT4070.15829000128343246No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3910.1520672985302754No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAGTGGTATCAACGCAG3860.15012270391991384No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3590.1396218930239613No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA3550.13806621733567204No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC3450.1341770281149489No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3410.13262135242665962No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3390.13184351458251498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTACAGAGATCGGAAGAGCA3370.13106567673837036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG3290.12795432536179183No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3280.1275654064397195No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA3260.12678756859557488No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3250.12639864967350256No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA3070.11939810907620088No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT3020.1174535144658393No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA2920.11356432524511616No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2890.11239756847889919No Hit
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA2870.11161973063475458No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCG2850.11084189279060995No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2710.10539702788159754No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2650.10306351434916364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT426350.064.398861
AGCAGTG454150.060.4492452
AGTGGTA460200.059.6230475
CAGTGGT467600.058.6794854
GCAGTGG469500.058.4728933
GTATCAA476050.058.003389
GGTATCA480450.057.4269148
TGGTATC479400.057.250267
GTGGTAT481000.057.0296866
TGCGGTA400.00604816854.369602145
GTGCATC450.00963483248.328537145
CAGGGGG2650.046.504818145
CATGCCG803.7290527E-545.308002145
CCGGGCT1505.329639E-1043.49568145
GTGTAGC907.471285E-540.27378145
AGGGGTA2800.038.835435
CACGGCT2450.038.465572145
CGCGGGC1001.389387E-436.246403145
CCGTCGT800.00203479836.246403145
AGTAGTC800.00203479836.246403145
Produced by FastQC (version 0.11.5)