FastQCFastQC Report
Sun 19 Mar 2023
SRR4896058_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896058_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703946
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT1705300.0143.881791
GCTGATC1705750.0143.843812
CTGATCG590650.0143.804953
TGATCGA162250.0143.467484
CTGATCA421600.0143.31693
CTGATCT531900.0143.309653
TGATCGT224000.0142.939294
TGATCGG193750.0142.847994
TGATCTA141100.0142.673284
GATCGAG55750.0142.579365
GATCGTA63050.0142.287065
GATCTAG56800.0142.225365
TGATCAG154650.0142.091174
GATCGAT58550.0142.032445
CTGATCC167650.0141.809723
TGATCAT147400.0141.655354
GATCGTG75950.0141.535235
TGATCTG204800.0140.94144
GATCATG62000.0140.74845
GATCGGT68900.0140.656045
Produced by FastQC (version 0.11.5)