FastQCFastQC Report
Sun 19 Mar 2023
SRR4896058_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896058_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703946
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG166380.9764393942061544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC118300.6942708278313985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT88060.5168004150366268No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49830.292438844892972No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG35370.20757700067959903No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG32320.18967737240499405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA24360.14296227697356606No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC23820.1397931624593737No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA18860.11068425877345878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC17500.10270278518215953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT552850.0137.831761
GGTATCA557800.0136.807858
GTGGTAT557450.0136.751686
TGGTATC559000.0136.48847
GTATCAA560850.0136.033349
AGCAGTG565700.0134.789832
CAGTGGT567100.0134.534124
AGTGGTA573550.0132.938055
GCAGTGG574900.0132.645343
TCAACGC556950.027.93498610-14
CAACGCA558650.027.87833610-14
AGAGTAC558200.027.77825415-19
ATCAACG558050.027.77412410-14
GTACATG559300.027.72844120-24
CAGAGTA559550.027.72152715-19
TACATGG561600.027.63026820-24
AGTACAT559950.027.5599620-24
CGCAGAG561650.027.53327215-19
GCAGAGT564650.027.45584115-19
ACATGGG566900.027.23732620-24
Produced by FastQC (version 0.11.5)