FastQCFastQC Report
Sun 19 Mar 2023
SRR4896057_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896057_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360553
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT113600.8349546103679901No Hit
CGGATTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATACCACT24850.18264632101799783No Hit
CGGATTGCGTTGATACCACTGCTTCCCATGTACGCTGCGTTGATACCACT19900.14626405586551938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTGC1362550.0143.788642
CGGATTG1363650.0143.672651
GATTGCG477750.0143.502673
GATTGCT427550.0143.292723
ATTGCGT201400.0142.820274
GATTGCA317950.0142.799823
ATTGCGA112500.0142.3364
GATTGCC145700.0142.023353
ATTGCTA116700.0140.976854
ATTGCGG155750.0140.948964
ATTGCTG149000.0140.52084
ATTGCAT114900.0140.177544
TTGCGTA44550.0140.121225
ATTGCCG47000.0139.251074
TTGCGAG34650.0138.805195
ATTGCCT52550.0138.382494
TTGCGAT39700.0137.652395
TTGCTAG36750.0137.338785
ATTGCTT162600.0136.383764
TGCGATA10200.0136.235296
Produced by FastQC (version 0.11.5)