FastQCFastQC Report
Sun 19 Mar 2023
SRR4896057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360553
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG375162.757408200930063No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG41020.30149505384942743No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33930.2493838902269886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT31470.2313030069390902No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA28500.20947364784760317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22730.16706442159915857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC20740.15243801601260665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG19980.1468520520700039No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAA19780.14538206155879263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC18370.13501862845475332No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGCTTCTCGGCCTTTTGG17740.1303881583444379No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC17160.12612518586192525No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA16810.12355270246730557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16510.1213477167004887No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC15290.11238077458210008No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGCTTCTCGGCCTTTTG14090.10356083151483257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT608000.099.9227146
AAGCAGT610450.099.756941
TGGTATC609300.099.733157
GGTATCA610200.099.586058
GTATCAA610350.099.51449
AGTGGTA612900.099.2178345
AGCAGTG620400.098.226512
CAGTGGT621900.097.909334
GCAGTGG631400.096.526653
CGACCTA6850.026.6975545-49
CATGGGG200600.025.87093725-29
ATGGGGG71250.024.15134425-29
ATGGGGC61650.023.75451525-29
ATGGGCG12850.023.64490525-29
TACGCAC7800.023.6305450-54
TCTCGCA8250.023.03977670-74
GATAAGT8800.022.90886795-99
CATGGGC65100.022.67259225-29
ATGGGAG65700.022.57512325-29
TGACGAC7950.022.4601640-44
Produced by FastQC (version 0.11.5)