FastQCFastQC Report
Sun 19 Mar 2023
SRR4896056_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896056_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457648
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACA1460800.0143.684561
GACACAC1465250.0143.248182
ACACACG451300.0141.798363
CACACGT162250.0141.514954
ACACACT448350.0141.18973
CACACTG144450.0139.962624
CACACTA131400.0139.780824
ACACGTA53400.0139.68545
CACACGA130150.0139.076454
CACACAT149600.0138.22464
ACACGAT47950.0138.14395
CACACGG153000.0137.694124
ACACGTG50600.0137.454545
CACACTT170500.0136.94784
ACACACA400750.0136.238563
ACACTAG41750.0136.067065
ACACGTT56650.0135.484545
ACACATG49150.0135.064095
ACACTTG57850.0134.665515
ACACTGA39850.0134.424095
Produced by FastQC (version 0.11.5)