FastQCFastQC Report
Sun 19 Mar 2023
SRR4896056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457648
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG43820.3006212748208072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC43300.29705388406528876No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC41920.2875865778294897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32450.22261890387802816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA29260.20073433366628982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28220.19359955215525285No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG27800.19071819808348792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTCAGGCGCAGTGGCTCAT26980.18509269727670877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT26180.17960440380668036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25800.17699746440841685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC25670.17610561671953723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA25520.1750765616939069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG23930.1641685784222254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCACGGTGGCTCAC22440.15394663183429744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAGGCTGGGCACGGTGGCT22400.15367221716079602No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC21050.14441072193012305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCATGCCT20950.1437246852463695No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGTGCAGTGGCTCAC20630.14152936785835812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC20270.13905963579684533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCCGGGCGCAGTGGCTC19210.13178764694905765No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCGCAGTGGCTC19180.13158183594393158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC19050.13068998825505196No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCACAGTGGCTCA18130.12437845076451928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA15270.10475780160916763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT14970.102699691557907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCAGGCGCGGTGGCTC14960.10263108788953162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGTCGTGGTGGCTTAC14740.1011218071852738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT422000.0136.831766
GGTATCA424400.0136.108868
AAGCAGT426750.0135.455471
TGGTATC426850.0135.411967
GTATCAA427100.0135.371069
AGTGGTA429850.0134.36645
AGCAGTG430250.0134.276642
CAGTGGT433600.0133.24214
GCAGTGG456750.0126.501863
CAACGCA421400.027.92567410-14
TCAACGC421150.027.90805810-14
TACATGG422150.027.71819520-24
GTACATG422100.027.7180720-24
AGAGTAC424300.027.64580715-19
CAGAGTA425100.027.60394515-19
AACGCAG426450.027.5645910-14
ATCAACG426100.027.53653710-14
AGTACAT425800.027.46706620-24
CGCAGAG427600.027.42908115-19
GCAGAGT428950.027.3528315-19
Produced by FastQC (version 0.11.5)