FastQCFastQC Report
Sun 19 Mar 2023
SRR4896055_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896055_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359797
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATC2365450.0143.652222
CCTCTAT2367450.0143.530871
CTATCGA210950.0143.282464
TCTATCG775000.0143.16313
CTATCGT299000.0143.084174
CTATCAG213750.0142.618184
TCTATCA621300.0142.585423
CTATCGG246050.0142.3024
CTATCTA205950.0141.726844
TATCGAG68650.0141.586995
TCTATCT754000.0141.526033
CTATCTG252600.0141.49094
CTATCAT222950.0141.480294
TATCGTA89850.0141.354835
TATCGAT75300.0141.13075
TCTATCC237850.0141.062913
CTATCCG74100.0140.889914
TATCGTG96750.0140.650395
TATCGAA64450.0140.424365
CTATCAA179550.0140.350114
Produced by FastQC (version 0.11.5)