FastQCFastQC Report
Sun 19 Mar 2023
SRR4896055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359797
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG162720.6895508384831407No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGTGCAGTGGCTCA145210.6153495406596414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCATGGTGGCTCA102510.4344017726948547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG98900.4191038466444359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC93000.3941016960357183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTGCAGTGGCTCAC74310.3148999680904756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG59750.2531997455713351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54390.23048592739121201No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC51210.21701019197837781No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGACGCGGTGGCTCACG49900.21145886701271335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC44020.18654146945690667No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC39690.16819243350169527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG34850.14768219469725574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACG32670.13844411192996686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT31160.13204525643519335No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCATGGTGGCTCAC28620.12128161871550816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC28310.11996794639538909No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCCGGGCGCGGTGGCT27530.1166625773318637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGTTGGCTCACG27010.11445899795618014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACGCGGTGGCTCACGCCT26320.11153501763075384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC24280.1028902062338413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA23960.10153415738726679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGTTGGCTCAC23870.1011527686491677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT780050.0133.493526
GGTATCA781350.0133.271428
AAGCAGT781550.0133.244311
TGGTATC784150.0132.859827
GTATCAA784400.0132.749669
AGTGGTA791300.0131.641135
CAGTGGT794350.0131.213734
AGCAGTG796650.0130.839742
GCAGTGG852850.0122.192513
CAACGCA775150.027.38878410-14
TCAACGC776600.027.33950210-14
TACATGG777450.027.21769720-24
GTACATG778250.027.2026720-24
AGAGTAC780050.027.1438215-19
ATCAACG780750.027.11671310-14
CAGAGTA782000.027.09823415-19
CGCAGAG785200.026.9327815-19
AACGCAG787750.026.92328310-14
AGTACAT784800.026.91508520-24
CATGGGG442000.026.85136825-29
Produced by FastQC (version 0.11.5)