FastQCFastQC Report
Sun 19 Mar 2023
SRR4896054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601213
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAATCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG181391.1328286742613256No Hit
CCTAATCCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG40800.25480682457611825No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC40190.25099721273809295No Hit
CCTAATCCATGTACTCTGCGGTGATACCACTGCTTCCCATGTACTCTGCG31860.1989741527204688No Hit
CCTAATCCGTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG22640.14139280657851266No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGC20490.12796548616580056No Hit
CCTAATCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC16850.10523272044381353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATC1604500.0143.702771
CTAATCC1605200.0143.64012
TAATCCG525750.0143.505923
TAATCCA447300.0142.646823
AATCCGA141150.0142.315644
AATCCGT200350.0142.058354
TAATCCT503800.0141.983863
ATCCATG119450.0141.708455
AATCCGG174950.0141.241614
ATCCGTG72950.0140.939335
ATCCGTA55750.0140.899415
AATCCTG174150.0140.650124
ATCCGGA44850.0140.627725
ATCCGAT50950.0139.900835
ATCCGGT62750.0139.86835
AATCCAT210400.0139.755634
ATCCGAG46750.0139.686685
AATCCTA149100.0139.55634
ATCCTAG49000.0139.155
AATCCAG125650.0139.013694
Produced by FastQC (version 0.11.5)