FastQCFastQC Report
Sun 19 Mar 2023
SRR4896054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1601213
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG804105.02181783435433No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG217411.3577831306640653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC113130.7065268643209867No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA92500.5776870410120327No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT53000.33099906133662416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC41900.26167661641518025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32000.19984848986362214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA26120.16312632985118158No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA24220.151260325765529No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC23760.14838750372373943No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCGGTGGCTCACG16380.10229744574894158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT993750.0101.267591
AGCAGTG1017750.098.886492
AGTGGTA1023900.098.2490545
CAGTGGT1030150.097.669994
GCAGTGG1037250.096.992743
GTGGTAT1048300.095.907286
TGGTATC1050100.095.763457
GGTATCA1050250.095.742928
GTATCAA1050450.095.6904149
CATGGGG193450.026.1050425-29
AAACCGC41250.026.0767885-89
GAAACCG42250.025.86856885-89
ATGGGAG149100.025.7960725-29
GATAAGT43600.024.86942995-99
CGCAGAT43750.024.81707690-94
GCATTGA43750.024.55376430-34
ATACAAC44350.024.48133130-134
CTACTTA44900.024.149378135-139
ATGGGGC55150.024.07369225-29
ACAACTA45500.023.989164130-134
Produced by FastQC (version 0.11.5)