FastQCFastQC Report
Sun 19 Mar 2023
SRR4896053_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896053_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003892
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC2008700.0143.652312
CCGACAA2011900.0143.423811
GACAACT637800.0143.243653
GACAACG674400.0142.97513
ACAACGT242850.0142.280414
GACAACA528200.0141.205613
CAACGTA73500.0140.375525
ACAACTG223250.0139.162374
ACAACTA191500.0139.037084
ACAACGA190600.0138.900314
ACAACGG237100.0138.625054
CAACTAG64950.0138.568135
CAACGTT87800.0138.013675
CAACGGT82450.0137.363255
CAACGTG80400.0137.104495
GACAACC191200.0136.958163
CAACGAT67450.0136.741295
CAACTAT68300.0136.409965
CAACTGT80550.0135.95535
ACAACAT201800.0135.187324
Produced by FastQC (version 0.11.5)