FastQCFastQC Report
Sun 19 Mar 2023
SRR4896053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003892
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG151910.758074786465538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC91610.4571603659279043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG74710.372824483555002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47430.23668940242288508No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT43630.21772630461122655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC28510.1422731364764169No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA25530.1274020755609584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC24860.12405858199942912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC24750.12350965022067056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG23440.11697237176454618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC22370.11163276264389499No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC21090.10524519285470474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA20490.10225101951602182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT608150.0133.413426
AAGCAGT608600.0133.395451
GGTATCA610400.0133.039588
TGGTATC612050.0132.645657
GTATCAA613300.0132.393639
AGTGGTA618900.0131.165885
CAGTGGT620300.0130.92284
AGCAGTG621150.0130.6312
GCAGTGG632350.0128.38243
TCAACGC608450.027.2057410-14
CAACGCA610150.027.14882310-14
AGAGTAC608450.027.12655415-19
GTACATG608500.027.07909220-24
ATCAACG610050.027.07537510-14
CAGAGTA610350.027.0515515-19
TACATGG608750.027.03722420-24
AGTACAT611850.026.86728920-24
CGCAGAG617100.026.72065215-19
GCAGAGT619950.026.63033115-19
GAGTACA608700.026.60652720-24
Produced by FastQC (version 0.11.5)