FastQCFastQC Report
Sun 19 Mar 2023
SRR4896052_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896052_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075200
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATCCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC122230.5890034695451041No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG91290.4399094063222822No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGATACC91000.43851195065535853No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGGACGCTGCGTTGATACC48800.23515805705474174No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG25870.12466268311488049No Hit
CCATCCTCTGCGTTGATACCACTGCTTCCCATGGACGCGGCGTTGATACC24810.11955474171164225No Hit
CCATCCTCATGTACTCTGCGGTGATACCACTGCTTCCCATGTACTCTGCG21210.10220701619121049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCTC2085700.0143.274722
ATCCTCG645700.0143.252563
CCATCCT2086450.0143.223221
ATCCTCA549250.0142.885423
ATCCTCT703100.0142.228093
TCCTCGA171000.0142.104934
CCTCGTA73400.0139.68365
TCCTCGT247350.0139.138554
TCCTCAG180750.0138.900914
TCCTCTA177550.0138.727924
CTCGAGT18550.0138.177586
CCTCGAT63850.0138.023165
TCCTCTG301800.0137.749194
TCCTCGG227300.0137.601094
CCTCTAG62050.0136.109275
CCTCGAA52600.0135.786765
CCTCGAG55600.0135.711915
CTCGTAG22400.0135.642556
CCTCAGT65300.0135.399385
CTCGTTA24600.0134.926516
Produced by FastQC (version 0.11.5)