FastQCFastQC Report
Sun 19 Mar 2023
SRR4896052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075200
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1256186.053296067848882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG129300.6230724749421742No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA82140.39581727062451816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC53610.25833654587509636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC43150.20793176561295298No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA39690.19125867386276021No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAC37550.18094641480339246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC37220.1793562066306862No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36720.176946800308404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC29620.1427332305319969No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT28640.13801079414032383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCGGTGGCTCACG28420.13695065535851966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA27890.13439668465690055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC25540.12307247494217424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC25240.12162683114880493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA23150.11155551272166538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG22620.10900154202004628No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA22590.10885697764070933No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG22360.10774865073245952No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC21860.10533924441017734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1195250.087.38281
AGTGGTA1228900.084.9818045
CAGTGGT1236750.084.481414
AGCAGTG1240950.084.172552
GTGGTAT1240850.084.163396
GTATCAA1241450.084.1363459
TGGTATC1242050.084.0994647
GGTATCA1242650.084.070458
GCAGTGG1278950.081.677263
CGACCTA9550.026.085445-49
CGATAGG8950.025.74256135-39
CATGGGG306600.025.48369625-29
AAACCGC26250.025.23383785-89
GAAACCG27250.024.519285-89
TATATCG3300.023.99957590-94
ATGGGAG111000.023.96065525-29
GATAAGT28350.023.87259395-99
ATGGGGC101450.023.76064325-29
ATGGGGG118450.023.43833525-29
ATGGGGT45350.023.4015725-29
Produced by FastQC (version 0.11.5)