FastQCFastQC Report
Sun 19 Mar 2023
SRR4896051_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896051_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11531834
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC11568750.0143.539962
CCTTACG3619550.0143.474753
CACCTTA11588450.0143.295961
CTTACGT1405500.0142.437454
CTTACGA983350.0142.35984
CTTACGG1172450.0142.102344
CCTTACA3087650.0141.97353
CCTTACT3922550.0141.852313
CTTACAG969450.0140.680074
CTTACTG1248450.0140.510774
CTTACTA1137550.0140.442764
TTACGAT381800.0138.870515
CTTACAT1217900.0138.868454
TTACGGA309200.0138.760575
TTACGTA414900.0138.654985
TTACGAG305250.0138.173845
CTTACCG316850.0137.932684
CCTTACC1072100.0136.625963
TTACGGT471150.0136.61885
TTACGTG453700.0135.636665
Produced by FastQC (version 0.11.5)