FastQCFastQC Report
Sun 19 Mar 2023
SRR4896051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11531834
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1463931.2694684991129772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC801770.6952666852471168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT392640.3404835692223804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG371430.3220910047785981No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG306500.2657859972663498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA271400.23534851438201418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA187700.16276682442705992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA168280.14592648489390325No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC162510.1409229442602105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCGCGGTAATTCCAGCTC138430.12004161697089985No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACAATACAGGACTCTTTCG122020.10581144334890703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAGGTAGTGACGAAAAA119540.10366087475764912No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTCTGGTGCCAGCAGCCG117100.1015449927565728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5154800.0137.917681
GGTATCA5201300.0136.640238
GTGGTAT5204050.0136.533436
TGGTATC5207100.0136.517067
GTATCAA5211150.0136.373469
AGCAGTG5253000.0135.344942
CAGTGGT5258550.0135.235554
AGTGGTA5272550.0134.79655
GCAGTGG5341650.0133.111653
TCAACGC5190400.027.88534210-14
CAACGCA5201100.027.83766710-14
ATCAACG5194850.027.76582310-14
AGAGTAC5201800.027.75083515-19
CAGAGTA5210500.027.7127915-19
GTACATG5209400.027.69769520-24
TACATGG5209550.027.68058820-24
CGCAGAG5227700.027.57175415-19
GCAGAGT5240150.027.55735815-19
AGTACAT5222900.027.55524620-24
ACATGGG5205100.027.46965620-24
Produced by FastQC (version 0.11.5)