FastQCFastQC Report
Sun 19 Mar 2023
SRR4896050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828642
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT84420.4616540580386976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG75060.41046853348003604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG66480.3635484693012629No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60420.3304091232728987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC45300.24772481437044538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG34130.18664123431486318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA27040.14786929316946673No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC23360.1277450698387109No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA22610.1236436656272797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA22530.1232061825113937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC21540.1177923289523045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA21060.11516743025698853No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC20550.11237847539321529No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC18290.1000195773694359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT602050.0135.350311
GTGGTAT606400.0134.262086
GTATCAA607250.0133.920039
TGGTATC608300.0133.878237
GGTATCA608000.0133.873258
AGCAGTG612700.0133.048892
AGTGGTA612350.0132.969285
CAGTGGT614850.0132.52234
GCAGTGG622950.0130.813463
CGTGGTA2151.9386789E-830.1391415
AGAGTAC604100.027.45999515-19
GTACATG604500.027.4275320-24
TCAACGC605550.027.42039910-14
CAACGCA606400.027.41995610-14
CAGAGTA606850.027.34267415-19
TACATGG606650.027.3065920-24
ATCAACG607150.027.24852810-14
AGTACAT607250.027.22981320-24
AACGCAG612400.027.13955310-14
GCAGAGT611000.027.13810715-19
Produced by FastQC (version 0.11.5)