FastQCFastQC Report
Sun 19 Mar 2023
SRR4896049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331063
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT2336600.0143.657971
TTGGCTC2347800.0142.972672
TGGCTCG710250.0142.793663
TGGCTCT799350.0142.378683
GGCTCGT267150.0142.167314
GGCTCTA226100.0142.089344
GCTCGTA72900.0141.925925
TGGCTCA578700.0141.710723
GGCTCAT219750.0141.673724
GGCTCGA199350.0141.616264
GCTCGAT74250.0141.381825
GCTCTAG74500.0141.197315
GGCTCTG277900.0140.580064
GCTCATA59600.0140.134235
GCTCATG79550.0140.01765
GCTCTAT85600.0139.373835
GCTCTGA64750.0138.996125
GCTCGGA59900.0138.951585
GGCTCAG189550.0138.834084
GGCTCGG235950.0138.354724
Produced by FastQC (version 0.11.5)