FastQCFastQC Report
Sun 19 Mar 2023
SRR4896049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331063
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG305541.3107324855655982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG184550.7916988944528741No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC101890.4370967236835727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC68120.2922271942028165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47790.20501376410676161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT39840.17090915174750748No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCCT39720.17039436514585835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA36140.1550365648633263No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA35570.15259132850549298No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC33960.14568460826670065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCTGGCGCGGTGGCTCA32420.1390781802122036No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCGGTGGCTCACGCCTG30870.13242885327423584No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG30320.13006941468334404No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG29410.1261656162875049No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT29340.12586532410320958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC28940.12414936876437917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC28470.12213312124125346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCACGGTGGCTC27470.11784323289417747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCTGTGGCTCAC25240.10827678188019801No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC24360.10450168013477112No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC23640.10141296052487642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT804400.0119.37231
GTGGTAT813650.0118.026376
GGTATCA815250.0117.794738
TGGTATC817600.0117.4825747
GTATCAA820900.0117.012829
AGTGGTA823250.0116.6850365
CAGTGGT828550.0116.0334
AGCAGTG828150.0115.995912
GCAGTGG850350.0112.9930343
CGACCTA10600.026.3543245-49
CATGGGG357300.026.14368825-29
CGATAGG5400.024.79962535-39
GATAAGT36900.024.7410995-99
TACATGG797300.024.57336620-24
GTACATG800550.024.46281420-24
TACGCAC11500.024.41702550-54
GAAACCG37250.024.39265385-89
AAACCGC37500.024.23003685-89
AGAGTAC810550.024.1930915-19
AGTACAT808650.024.16969920-24
Produced by FastQC (version 0.11.5)