FastQCFastQC Report
Sun 19 Mar 2023
SRR4896048_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896048_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771986
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAAGT1775050.0143.748322
CATCAAG1775300.0143.728071
TCAAGTA442300.0143.08823
TCAAGTG664800.0142.646013
CAAGTAG170700.0141.595584
CAAGTCG68150.0141.569874
TCAAGTT497650.0141.496843
TCAAGTC188500.0141.249683
CAAGTGT235850.0141.099664
CAAGTAT142050.0141.009314
CAAGTTG210400.0140.475084
CAAGTGG252050.0140.257694
CAAGTGA172150.0138.520864
CAAGTAA129000.0137.916084
CAAGTTA135800.0137.319414
CAAGTCT56300.0135.303544
AAGTCGA14700.0135.183475
AAGTTAG62350.0133.491415
AAGTCGT21400.0130.878335
CAAGTTT158250.0130.851014
Produced by FastQC (version 0.11.5)