FastQCFastQC Report
Sun 19 Mar 2023
SRR4896047_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896047_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107240
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG72310.6530652794335465No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC61270.5533578989198368No Hit
ATCATTCCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCG25150.22714136049998193No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCCTCCCATGTACTCTGC21590.19498934287056102No Hit
ATCATTCCATGTACTCTGCGGTGATACCACTGCTTCCCATGTACTCTGCG20130.18180340305624798No Hit
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC14470.13068530761171923No Hit
ATCATTCCATGTACTCTGCGGTGATACCACTGCTCCCCATGTACTCTGCG11760.10621003576460387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC1110000.0143.636751
TCATTCC1111250.0143.475192
CATTCCG322550.0143.419633
CATTCCT369700.0142.149863
ATTCCGA86050.0141.90824
ATTCCGT130800.0141.633034
ATTCCGG101800.0141.453834
CATTCCA297550.0141.386663
ATTCCTA110300.0140.540344
ATTCCTG115650.0139.392994
ATTCCAT140850.0138.274774
TCCGATA9200.0137.739146
ATTCCAG78500.0137.212744
TTCCGTA36550.0136.711355
TCCGTAT13150.0135.787066
CATTCCC137300.0135.137653
TTCCGGA25150.0133.98015
TTCCGAG28500.0133.894745
TCCGTAG11850.0133.67096
ATTCCCG32000.0132.754
Produced by FastQC (version 0.11.5)