FastQCFastQC Report
Sun 19 Mar 2023
SRR4896047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107240
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG578885.228134821718868No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG147531.3324121238394568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC84240.7608106643546114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA72490.65469094324627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGTCTCGGTGGCTCATG50770.45852750984429747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGTGCAGTGGCTCA48590.4388389147790903No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGTCTCGGTGGCTCAT40180.3628842888623966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCAGTGGCTCACG34450.3111339908240309No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT32610.29451609407174595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA30470.27518875763158845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTGGGGCACAGTGGCTCA29380.2653444600989849No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCAGTGGCTCAC28620.25848054622304106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGGGGCACAGTGGCTCAC24640.2225353130305986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA19490.17602326505545318No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18710.16897872186698457No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGTGCAGTGGCTCAC18380.16599833821032478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG18340.1656370795852751No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC18230.1646436183663885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTGGGGCACAGTGGCTCACA17580.1587731657093313No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCCAGGCACGGTGGCTC17140.1547993208337849No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCACGGTGGCTCA16520.14919981214551498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT15070.13610418698746435No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGCAGCTCTTAGTCGCGGG12170.10991293667136302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT679950.093.717751
AGCAGTG697200.091.502392
TGGTATC698450.091.105657
GGTATCA698600.091.0551768
GTGGTAT698950.091.0198756
CAGTGGT700500.090.9459154
GTATCAA699700.090.922329
AGTGGTA701750.090.75935
GCAGTGG720200.088.530213
CATGGGG168450.026.83347725-29
GAAACCG30600.026.02317885-89
GATAAGT31350.025.76807295-99
ATGGGGG71350.025.71177725-29
AAACCGC30600.025.59965585-89
ATGGGCG10400.025.4765825-29
CGCAGAT31700.025.1655990-94
GCATTGA31650.024.93236530-34
ATGGGGC43500.024.72794225-29
GCAGATA32250.024.60245990-94
ATACAAC32350.024.570919130-134
Produced by FastQC (version 0.11.5)