FastQCFastQC Report
Sun 19 Mar 2023
SRR4896046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254111
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG198540.8807906975299796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC123610.5483758341980497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC56370.2500764159351514No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55020.2440873586083383No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG54380.24124810180155282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC49630.2201754926886919No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC42380.18801203667432526No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA41540.18428551211541935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG39630.17581210508266895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG35680.15828856697828989No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC35000.15527185662108034No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC33160.1471089933015721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC31470.13961158079615424No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCTGGCGCGGTGGCTCA31050.13774831851670127No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCAGTGGCTCA29870.13251343877919056No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT29620.13140435408903997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT658200.0135.662176
GGTATCA661750.0134.945288
AAGCAGT662500.0134.853871
TGGTATC664050.0134.55387
GTATCAA665400.0134.292719
CAGTGGT676050.0132.192614
AGCAGTG675900.0132.163882
AGTGGTA675700.0132.1385
GCAGTGG694400.0128.601353
TCAACGC657000.027.72581510-14
CAACGCA658800.027.66536310-14
GTACATG660450.027.48478120-24
ATCAACG660450.027.47413810-14
TACATGG660850.027.4594320-24
AGAGTAC661650.027.45936215-19
CAGAGTA663750.027.39417615-19
AGTACAT663650.027.26763320-24
CGCAGAG665950.027.25178715-19
GCAGAGT671550.027.0781415-19
GAGTACA659650.027.04659820-24
Produced by FastQC (version 0.11.5)