FastQCFastQC Report
Sun 19 Mar 2023
SRR4896045_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896045_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375169
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTAAC2378200.0143.815022
GCTAACG721950.0143.78033
AGGCTAA2379800.0143.718341
GCTAACT806800.0143.696293
GCTAACA626300.0143.068533
CTAACGT262450.0143.039524
CTAACTG278350.0142.835714
CTAACGA208000.0142.718934
CTAACGG242050.0142.512424
GCTAACC229750.0142.244753
CTAACTA229300.0141.895924
TAACGTA79250.0141.18335
CTAACTT291700.0140.815614
TAACGTG83800.0140.30525
TAACGAT75600.0140.094945
CTAACAT231150.0139.888094
TAACGAG64800.0139.333055
CTAACCG73300.0139.186614
TAACTAG82400.0138.494865
TAACGTT95350.0137.581255
Produced by FastQC (version 0.11.5)