FastQCFastQC Report
Sun 19 Mar 2023
SRR4896045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375169
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG155050.6527956536987474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC77410.3259136507760079No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC69430.2923160415111514No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66010.2779170661119272No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG63640.2679388287738683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT57890.24373002510558198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC45550.191775827320077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA43400.18272383986150037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC38520.1621779334438939No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT34300.14441077666473418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC30800.1296749831275164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC28410.11961254125495913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG25070.10555038399372844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT24340.10247691848453731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT788600.0135.990546
AAGCAGT788850.0135.989441
GGTATCA791550.0135.365468
TGGTATC793700.0135.171147
GTATCAA793950.0135.004469
CAGTGGT804050.0133.489994
AGCAGTG803350.0133.485922
AGTGGTA806250.0133.049225
GCAGTGG815350.0131.598043
CAACGCA787500.027.74867410-14
TCAACGC787250.027.73004710-14
GTACATG791500.027.51871120-24
ATCAACG791500.027.50837710-14
TACATGG792350.027.44739320-24
AGAGTAC794050.027.43952415-19
CAGAGTA795550.027.40769815-19
AGTACAT794750.027.3572620-24
CGCAGAG796300.027.3493315-19
GCAGAGT802250.027.19854515-19
GAGTACA789800.027.12578820-24
Produced by FastQC (version 0.11.5)