FastQCFastQC Report
Sun 19 Mar 2023
SRR4896044_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896044_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6623772
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATGC6634200.0143.773072
AGCCATG6635400.0143.747071
CCATGCG2153250.0143.578583
CATGCGA606700.0143.062384
CATGCGT765100.0142.898864
CCATGCA1735850.0142.755553
CCATGCT2037550.0142.681823
CATGCGG740200.0142.258744
ATGCGAT220200.0141.41685
CATGCTG666050.0141.41634
CATGCAG589000.0141.249474
CATGCAT632600.0141.131734
ATGCGTA224000.0140.689185
CATGCTT756600.0140.355164
ATGCGAG194550.0140.002985
CATGCAA504300.0139.48834
ATGCTAG194950.0139.013995
CCATGCC764700.0138.793153
ATGCGAA184250.0138.685385
ATGCGGT265850.0138.420825
Produced by FastQC (version 0.11.5)