FastQCFastQC Report
Sun 19 Mar 2023
SRR4896043_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896043_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692084
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG48080.2841466499299089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCT1695900.0143.67311
GCACCTC1696900.0143.588422
CACCTCG514550.0142.992543
ACCTCGA147950.0142.686054
CACCTCT548350.0142.056723
ACCTCGT181400.0141.936054
ACCTCAT190650.0141.771844
CACCTCA484400.0141.502883
ACCTCGG176750.0140.618944
ACCTCAG151700.0140.392884
CCTCGTA53650.0140.376515
ACCTCTA162050.0140.223394
CCTCGAT54750.0139.791785
ACCTCTG178050.0139.228324
CTCGAAT15100.0138.754966
CCTCTAG56800.0137.661975
CCTCAGT52400.0136.717565
CTCGATG15800.0136.708866
CCTCGAA45100.0136.656335
ACCTCTT210900.0136.557624
Produced by FastQC (version 0.11.5)