FastQCFastQC Report
Sun 19 Mar 2023
SRR4896043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692084
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG196341.1603442855082846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG150520.889553946494382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC78240.46238839206564214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55230.3264022353500181No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG44290.2617482347212077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT32590.19260273130648362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAT23740.1403003633389359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC22730.13433139253134005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC20420.12067958801099708No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGCGACCTCAGATCAGACGT18260.10791426430366341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT526850.0121.519731
GGTATCA534050.0119.893038
GTGGTAT534450.0119.789846
TGGTATC535050.0119.65557
GTATCAA537050.0119.276949
CAGTGGT538550.0118.961614
AGCAGTG540000.0118.592342
AGTGGTA540100.0118.563365
GCAGTGG550850.0116.2956543
CGACCTA3750.025.72770945-49
AAACCGC28650.025.3819185-89
CATGGGG215100.025.31184825-29
GAAACCG28900.025.31182785-89
TACATGG525750.024.72417320-24
ATGGGAG91200.024.71024925-29
CGCAGAT29600.024.66458590-94
GTACATG531650.024.4931320-24
ATCAACG534150.024.40006310-14
AGTACAT532250.024.39517820-24
TCAACGC536250.024.37969810-14
Produced by FastQC (version 0.11.5)