FastQCFastQC Report
Sun 19 Mar 2023
SRR4896041_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896041_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581033
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTC3589400.0143.6632
AGATTCG1152500.0143.662633
ACAGATT3593700.0143.49111
GATTCGA319950.0143.054864
AGATTCA958700.0142.798393
GATTCGT447550.0142.536034
GATTCGG367350.0142.41244
AGATTCT1182300.0141.338753
GATTCTA341700.0141.260764
GATTCAG323850.0141.065324
GATTCAT357550.0141.039864
ATTCGTA131000.0140.647325
AGATTCC328350.0140.359993
ATTCGAG102050.0140.190115
ATTCGAT121550.0140.09055
ATTCGAA94700.0139.514255
GATTCAA278200.0138.90154
ATTCGGA101450.0138.890095
ATTCGTG147700.0138.296545
GATTCTG374100.0137.841224
Produced by FastQC (version 0.11.5)