FastQCFastQC Report
Sun 19 Mar 2023
SRR4896041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581033
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG233920.6532193364316944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT135100.37726544268092477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC123840.3458220016403088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG117020.3267772176352466No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113760.3176736991812139No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC81260.22691776367321942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA62620.17486574404648045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG59450.16601354972154683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC53990.15076655255620375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC49100.13711127487515473No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC42140.1176755422248273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC42050.11742421809572824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC38250.10681275486710119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT977900.0133.947596
GGTATCA979100.0133.827558
AAGCAGT980000.0133.728971
GTATCAA982250.0133.378259
TGGTATC983350.0133.256477
AGCAGTG995300.0131.691352
CAGTGGT997700.0131.421174
AGTGGTA999900.0131.029255
GCAGTGG1013150.0129.364073
CGTGGTA3400.029.6465515
TCAACGC975700.027.3978410-14
CAACGCA981850.027.2482310-14
AGAGTAC978950.027.24185815-19
CAGAGTA979550.027.22517215-19
GTACATG977900.027.22391120-24
TACATGG977700.027.21180720-24
ATCAACG979800.027.17884610-14
AGTACAT982050.027.04581520-24
CGCAGAG990400.026.8847515-19
GCAGAGT993500.026.83419815-19
Produced by FastQC (version 0.11.5)