FastQCFastQC Report
Sun 19 Mar 2023
SRR4896040_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896040_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127910
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC1130300.0143.694241
CACGACC1131450.0143.54822
ACGACCG380750.0143.413793
ACGACCT346650.0143.356113
CGACCGT132950.0143.24184
ACGACCA293450.0143.214863
CGACCTA103500.0143.095644
CGACCAT104000.0142.892324
CGACCGA106350.0142.849084
CGACCTG114650.0142.434
GACCGTA39950.0142.197745
GACCGAT36800.0141.652185
CGACCTT122850.0141.597084
GACCTAG35550.0141.164555
CGACCAG104250.0140.684894
CGACCGG134700.0140.52564
GACCTAT36750.0140.277545
CGACCAA83850.0140.135964
GACCGGT47750.0139.778025
GACCGTG45650.0139.741525
Produced by FastQC (version 0.11.5)