FastQCFastQC Report
Sun 19 Mar 2023
SRR4896040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127910
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT57470.5095264693104946No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43940.3895700898121304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG35920.31846512576358044No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA34340.30445691588867907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG25200.22342208154905976No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23480.2081726378877747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC19240.17058098607158373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC18130.16073977533668465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA16870.14956867125923168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG15930.14123467297922707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGTGCAGTGGCTCAC15820.14025941786135418No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC15200.13476252537879796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT14740.1306841857949659No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGCCGTGGTGGCTCAC14390.12758110132900677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC14180.12571925064943124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA13980.12394605952602601No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC12300.10905125408942204No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGCCGTGGTGGCTCACG12040.10674610562899522No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC11940.1058595100672926No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGTGTAGTGGCTCACG11620.10302240426984421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT286900.0133.73376
GGTATCA287050.0133.638738
AAGCAGT287050.0133.562731
TGGTATC287950.0133.196037
GTATCAA288300.0132.990289
AGTGGTA290900.0131.919565
AGCAGTG292100.0131.41392
CAGTGGT292800.0131.099734
GCAGTGG304750.0125.958993
CGACCTA6550.027.92017445-49
AGAGTAC286250.027.1699115-19
CAGAGTA286900.027.11839315-19
TCAACGC287400.027.10223810-14
ATCAACG287400.027.06716310-14
CAACGCA288800.027.02570310-14
GTACATG288100.026.97021120-24
TACATGG288450.026.94747220-24
TATATCG2200.026.83594390-94
AGTACAT289350.026.8089120-24
GCAGAGT291700.026.67215315-19
Produced by FastQC (version 0.11.5)