FastQCFastQC Report
Sun 19 Mar 2023
SRR4896039_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896039_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268976
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC2276450.0143.525572
AATGTTG2283350.0143.091871
TGTTGCG742500.0142.235153
TGTTGCA572100.0141.747243
GTTGCGA192500.0141.419224
GTTGCTA203150.0140.987444
GTTGCGT273750.0140.975344
GTTGCAT205550.0140.707374
GTTGCAG195750.0139.917244
TGTTGCT764950.0139.642063
GTTGCGG268350.0139.492464
TTGCGAG66550.0138.049595
TTGCGTA80400.0138.05
GTTGCTG265000.0137.750954
TTGCGAT72200.0137.5185
GTTGCAA171750.0135.783424
TTGCTAG74150.0135.358055
TTGCGGA67850.0134.555655
GTTGCTT294800.0133.400284
TGCGATA19850.0133.11846
Produced by FastQC (version 0.11.5)