FastQCFastQC Report
Sun 19 Mar 2023
SRR4896039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268976
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG110770.48819379314721706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT75960.33477656881342066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70370.31013990452080586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC67180.2960806989584729No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG52240.23023601836246835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA51560.22723907172222182No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGACAAACCTACCGAGCC22730.10017734872471105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT646050.0135.284181
GTGGTAT648250.0134.779166
GGTATCA650100.0134.428838
TGGTATC650450.0134.389717
GTATCAA650600.0134.259129
AGTGGTA660150.0132.425955
CAGTGGT660850.0132.387424
AGCAGTG663550.0131.822752
GCAGTGG672250.0130.084613
CGTGGTA1701.9608706E-938.1170275
TCAACGC647600.027.54322410-14
AGAGTAC648200.027.51550515-19
CAGAGTA649100.027.470715-19
GTACATG647950.027.45278520-24
CAACGCA651350.027.42444210-14
ATCAACG648350.027.38920610-14
AGTACAT649400.027.2872720-24
TACATGG652450.027.27447720-24
CGCAGAG653400.027.17751915-19
GCAGAGT657700.027.08522215-19
Produced by FastQC (version 0.11.5)