FastQCFastQC Report
Sun 19 Mar 2023
SRR4896038_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896038_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158835
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC1159800.0143.875232
AATCCGT1160550.0143.782261
TCCGTCG376500.0143.234473
CCGTCGA103250.0143.16264
CGTCGAT40150.0143.102775
TCCGTCT367400.0142.431633
CCGTCGT141100.0142.417564
CCGTCTA107950.0142.331974
CGTCGAA29900.0142.313785
CCGTCAG104350.0142.205444
TCCGTCA310750.0142.122653
CCGTCTG113650.0141.908784
CGTCATA33650.0141.43185
CGTCAGT36000.0141.399415
CCGTCAA88800.0141.323734
CGTCAAT32200.0141.316185
CGTCTAT38900.0141.223055
TCCGTCC116600.0141.035423
CGTCGTA38750.0141.02655
CCGTCGG126300.0140.521974
Produced by FastQC (version 0.11.5)