FastQCFastQC Report
Sun 19 Mar 2023
SRR4896038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158835
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG62300.5376088916886356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGGCGCGGTGGCTCAC60900.5255277929990033No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT54180.4675385192887685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC47360.4086863099578456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46820.40402645760613026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGGCGCGGTGGCTCACG31060.26802780378569857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGAGAGAGGCACTTTGCA28900.24938839437883736No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA23170.19994218331341387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG22250.1920031756030841No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA21240.18328752583413516No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA19140.16516587779968675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCTTTCCGGCGGTGACGAC16150.139364102741115No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGTGGTGGCTCA14820.12788705898596434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGAGAGAGGCACTTTGC14330.12365867444459307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC14170.12227797745149223No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC12470.10760807189979592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA329650.0135.741568
GTGGTAT329950.0135.596336
TGGTATC330800.0135.313227
GTATCAA331500.0134.984029
AGCAGTG336200.0133.124162
AAGCAGT336750.0132.786541
CAGTGGT337050.0132.718614
AGTGGTA337250.0132.618555
GCAGTGG341550.0131.038913
CAACGCA329600.027.78591510-14
TCAACGC329450.027.77234310-14
GTACATG329600.027.66358820-24
AGAGTAC330650.027.63671115-19
ATCAACG329950.027.61678710-14
CAGAGTA330950.027.60730715-19
TACATGG331250.027.5431820-24
AGTACAT331650.027.40141720-24
CGCAGAG333300.027.3089715-19
GCAGAGT335200.027.24868415-19
GAGTACA329950.027.07562320-24
Produced by FastQC (version 0.11.5)